Optimization of phenol-chloroform RNA extraction

Modified acid guanidinium thiocyanate-phenol-chloroform RNA extraction method which greatly reduces DNA contamination.

The most common and consistently successful methods for isolating intact RNA are modifications of the Guanidinium Thiocyanate method of Chirgwin et al. (1). One widely used modification of this method, developed by Chomczynski and Sacchi (2), involves extraction of the guanidinium thiocyanate homogenate with phenol—chloroform at reduced pH. The Acid Guanidinium Thiocyanate Phenol -Chloroform (A...

Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation†

Optimization of RNA Extraction from Rat Pancreatic Tissue

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...

Optimization of phenol and flavonoid extraction compound of Adiantum capillus-veneris L. using ultrasound assisted extraction

Background: Antioxidants are molecules which able to control free radicals and prevent the oxidants of other molecules considering undesirable effects of synthetic anti oxidant. In recent years using of natural anti oxidant gets the great importance. The secondary metabolisms of plants are rich sources of phenol and flavonoids considered as the most important natural antioxidant.  Objective: In...

An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.